CST, 2320S, AP-2 gamma Antibody

Polyclonal Antibody for studying AP-2gamma. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, AP-2 gamma Antibody (CST #2320) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity AP-2 gamma Antibody detects endogenous levels of total AP-2γ protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human AP-2γ. Antibodies are purified by protein A and peptide affinity chromatography. Background Activator protein 2γ (AP-2γ) is a member of the developmentally-regulated transcription factor activator protein 2 family (1). Overexpression of p53 in human breast cancer cells increases AP-2γ mRNA and protein (2). In addition, p53 binds to the AP-2γ promoter, suggesting that AP-2γ is a target of p53. AP-2γ therefore may contribute to p53-mediated growth inhibition (2). Studies also found that AP-2γ increases p21 mRNA and protein levels and induces cell-cycle arrest in G1 phase (3). Overexpression of AP-2γ inhibits the growth of human breast cancer cells (3). Taken together, these findings suggest that AP-2γ functions as a tumor suppressor (3). Alternate Names Activating enhancer-binding protein 2 gamma; AP-2 gamma; AP2-gamma; AP2C; ERF1; estrogen receptor factor 1; hAP-2g; TFAP2C; TFAP2G; Transcription factor AP-2 gamma; transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma); transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma); Transcription factor ERF-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit