{"product_id":"cst-2325t","title":"CST,  2325T, Phospho-MAPK\/CDK Substrates (PXS*P or S*PXR\/K) (34B2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying . Validated for WB,IP,IHC,E. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MAPK\/CDK Substrates (PXS*P or S*PXR\/K) (34B2) Rabbit Monoclonal Antibody (CST #2325) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:200\nPeptide ELISA (DELFIA): 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-MAPK\/CDK Substrates (PXS*P or S*PXR\/K) (34B2) Rabbit Monoclonal Antibody detects phospho-serine in a PXS*P or S*PXR\/K motif, as well as a PXS*PXR\/K motif. This antibody is phospho-specific, and does not react with phospho-threonine- or phospho-tyrosine-containing peptides\/proteins.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK\/CDK substrate peptides .\n\u003cb\u003eBackground\u003c\/b\u003e\nThe MAPK and CDK families of serine\/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S\/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S\/T)PXR\/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*\/T*P and\/or S*PXR\/K, for use in the study and discovery of new MAPK and CDK substrates (4,5).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800201023657,"sku":"2325T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2325t","provider":"Iright","version":"1.0","type":"link"}