CST, 23303S, USP39 (E8U2M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying USP39. Validated for WB,IP,IHC,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, USP39 (E8U2M) Rabbit Monoclonal Antibody (CST #23303) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity USP39 (E8U2M) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP39 protein. This antibody may detect an 80 kDa band of unknown origin by western blotting. Cytoplasmic staining of uncertain specificity was observed in human brain by immunohistochemistry. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala367 of human USP39 protein. Background Ubiquitin specific protease 39 (USP39) is a 65 kDa protein that plays an important role in pre-mRNA splicing, as well as mitotic spindle formation. It displays significant homology with ubiquitin C-terminal hydrolase proteins (UCHs), containing both an N-terminal zinc finger domain as well as UCH-1 and UCH-2-like domains also observed in the UCH2 family of proteins (1). However, USP39 lacks a catalytic cysteine residue found in UCHs and has been shown experimentally to lack peptidase activity (2). USP39 associates with the U4/U6-U5 tri-small nuclear ribonucleoprotein (U4/U6-U5 tri-snRNP) complex and is necessary for the formation of the mature spliceosome. Silencing of USP39 has been shown to adversely affect chromosome segregation and cytokinesis in U-2 OS cells, likely due to improper splicing of Aurora B and other mRNAs necessary for mitotic spindle formation and checkpoint function (2). In addition, USP39 has been found to be overexpressed in many types of cancers, and in most cases is associated with tumor progression and poor prognosis. Overexpression has been observed in pancreatic (3), prostate (4), colorectal (5,6), lung (6,7), gastric (8), and triple negative breast cancers (9), as well as melanoma (10) and hepatocellular carcinoma (11,12). Alternate Names 65K; CGI-21; HSPC332; Inactive ubiquitin-specific peptidase 39; MGC75069; SAD1; SAD1 homolog; small nuclear ribonucleoprotein 65kDa (U4/U6.U5); SnRNP assembly defective 1 homolog; SNRNP65; SNUT2; U4/U6.U5 tri-snRNP-associated 65 kDa protein; U4/U6.U5 tri-snRNP-associated protein 2; Ubiquitin carboxyl-terminal hydrolase 39; ubiquitin specific peptidase 39; ubiquitin specific protease 39; USP39 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG