{"product_id":"cst-2331s","title":"CST,  2331S, Phospho-eEF2 (Thr56) Antibody","description":"Polyclonal Antibody for studying eEF2 (Thr57) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-eEF2 (Thr56) Antibody (CST #2331) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-eEF2 (Thr56) Antibody detects endogenous levels of eEF2 only when phosphorylated at Thr56. This antibody does not recognize eEF2 phosphorylated at other sites.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Chicken\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr56 of human eEF2. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nEukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5,6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A) (10). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nEEF-2; EEF2; EF-2; EF2; Elongation factor 2; epididymis secretory sperm binding protein; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase; SCA26\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk C\nSENSITIVITY: Endogenous\nMW (kDa): 95\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797665665193,"sku":"2331S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2331s","provider":"Iright","version":"1.0","type":"link"}