CST, 23369T, ID1 (F2M1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ID1. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, ID1 (F2M1J) Rabbit Monoclonal Antibody (CST #23369) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 70322 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity ID1 (F2M1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total ID1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human ID1 protein. Background Inhibitor of DNA-binding/Differentiation (ID) proteins are a family of proteins that function to repress the activity of basic helix-loop-helix (bHLH) transcription factors. There are four known ID proteins in humans (ID1-4), all of which contain a helix-loop-helix domain but lack a basic DNA-binding domain. Heterodimerization with bHLH transcription factors, therefore, functions to sequester bHLH proteins and prevent their binding to DNA (1). ID proteins play important functional roles in development, primarily by inhibiting premature differentiation of stem/progenitor cells (1,2). ID1 plays important regulatory roles downstream of TGF-β and BMP signaling by inhibiting various transcription factors (3,4). SMAD3 is capable of binding to the ID1 promoter, causing upregulation in response to TGF-β (5). High expression of ID1 is associated with increased cancer growth and migration by inhibiting cell cycle regulators p16, p21, and p27 (6,7). ID1 as a therapeutic target is context dependent. While high expression of ID1 is generally associated with poor prognosis, drug sensitivity can vary greatly depending on tumor type (8-10). Alternate Names BHLHB24; Class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein); DNA-binding protein inhibitor ID-1; ID; ID1; inhibitor of differentiation 1; Inhibitor of DNA binding 1; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein; inhibitor of DNA binding 1, HLH protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG