CST, 23408S, ACAA2 (F4Y9U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MKT/ACAA2. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ACAA2 (F4Y9U) Rabbit Monoclonal Antibody (CST #23408) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ACAA2 (F4Y9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACAA2 protein. This antibody does not cross-react with ACAA1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val219 of human ACAA2 protein. Background Acetyl-CoA acyltransferase 2 (ACAA2), also known as medium-chain 3-ketoacyl-CoA thiolase, is a mitochondrial enzyme that participates in the catalyzation of the last step in the fatty acid β-oxidation pathway (1,2). Studies identified a short-chain carbon circuit at the C4 node of fatty acid oxidation (FAO) where reverse flux of acetyl-CoA derived from pyruvate through ACAA2 produces free CoA and NAD , therefore promoting lipid tolerance and redox stability in heart mitochondria (3). Alternate Names 3-ketoacyl-CoA thiolase, mitochondrial; ACAA2; Acetyl-CoA acetyltransferase; Acetyl-CoA acyltransferase; acetyl-CoA acyltransferase 2; acetyl-Coenzyme A acyltransferase 2; Acyl-CoA hydrolase, mitochondrial; beta ketothiolase; Beta-ketothiolase; DSAEC; FLJ35992; FLJ95265; Mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase; T1; THIM Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG