CST, 23451S, CD200 (E5I9V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD200. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, CD200 (E5I9V) Rabbit Monoclonal Antibody (CST #23451) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 24510 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD200 (E5I9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD200 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human CD200 recombinant protein and reacts with an epitope surrounding Ala164. Background CD200 (OX2) and CD200R (OX2R) are membrane glycoprotein members of the Ig superfamily (1-3). CD200 is expressed by a range of cells, including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells, while its receptor, CD200R, is found on myeloid and T cells (1-5). Interaction between CD200 and CD200R downregulates macrophage function and plays a role in immunosuppression and regulation of anti-tumor immune responses (3-7). Alternate Names antigen identified by monoclonal antibody MRC OX-2; CD200; CD200 antigen; CD200 molecule; MOX1; MOX2; MRC; MRC OX-2 antigen; OX-2; OX-2 membrane glycoprotein; OX2G Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-50 Source/Isotype: Rabbit IgG