CST, 23542T, EXOSC2 (F6Y3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RRP4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, EXOSC2 (F6Y3J) Rabbit Monoclonal Antibody (CST #23542) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EXOSC2 (F6Y3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total EXOSC2 protein. This antibody detects a 90 kDa protein of unknown origin. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu14 of human EXOSC2 protein. Background Exosome component 2 (EXOSC2), also known as RRP4, is part of the exosome complex involved in RNA processing and mRNA decay by utilizing 3'-5' exoribonuclease activity (1,2). The exosome consists of an inert ring of nine subunits coupled with the enzyme DIS3 or DIS3L (3-5). EXOSC2, along with EXOSC1 and EXOSC3, forms the cap of the complex and assists in 5.8S rRNA generation and mRNA degradation (1,6). Missense mutations in alter exosome binding to certain mRNAs and cause a syndrome termed SHRF (short stature, hearing loss, retinitis pigmentosa, and distinctive facies) (7,8). Overexpression of EXOSC2 was shown to promote metastatic breast cancer in a tRNA-dependent manner (9). Alternate Names EXOS2; EXOSC2; Exosome complex component RRP4; Exosome complex exonuclease RRP4; Exosome component 2; homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP4); homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease; hRrp4p; p7; Ribosomal RNA-processing protein 4; RRP4; Rrp4p; SHRF Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG