CST, 23816S, Phospho-TFE3 (Ser321) Antibody

Polyclonal Antibody for studying TFE3 (Ser321) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-TFE3 (Ser321) Antibody (CST #23816) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-TFE3 (Ser321) Antibody recognizes endogenous levels of TFE3 protein only when phosphorylated at Ser321. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser321 of human TFE3 protein. Antibodies are purified by peptide affinity chromatography. Background Transcription factor E3 (TFE3) is a member of a family of basic helix-loop-helix leucine zipper transcription factors that include MITF, TFEB, TFE3, and TFEC. Members of this family form heterodimers with each other, bind the same DNA sequences, and undergo the same types of post-translational modifications, including sumoylation (1). Research studies indicate that TFE3 and other family members play roles in development, organelle biogenesis, nutrient sensing, autophagy, and energy metabolism (2,3). Additional studies report that TFE3 controls the gate for pluripotent cells to exit the state of pluripotency prior to differentiation (4). Translocations involving the gene region have been identified in a number of tumors, including sporadic renal cell tumors. Several specific translocations that result in kidney cancer and involve the gene have been described and characterized in detail (5). Phosphorylation of TFE3 at Ser321 by mTOR leads to cytoplasmic retention analogous to Ser211 on TFEB (6). Dephosphorylation of TFEB and TFE3 by protein phosphatase 2A in response to oxidative stress leads to their nuclear translocation and activation (7). Alternate Names BHLHE33; class E basic helix-loop-helix protein 33; DKFZp761J1810; Putative uncharacterized protein DKFZp761J1810; RCCP2; RCCX1; TFE3; TFEA; transcription factor binding to IGHM enhancer 3; transcription factor E family, member A; Transcription factor E3; Transcription factor for IgH enhancer; transcription factor for immunoglobulin heavy-chain enhancer 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 76-90 SOURCE: Rabbit