CST, 23827S, IDE (F4P1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IDE. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, IDE (F4P1J) Rabbit Monoclonal Antibody (CST #23827) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IDE (F4P1J) Rabbit mAb recognizes endogenous levels of total IDE protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human IDE protein. Background Insulin-degrading enzyme (IDE) is a highly conserved zinc metalloprotease responsible for clearing short bioactive peptides, including insulin, glucagon, amylin, and β-amyloid (Aβ). IDE has the highest affinity for insulin and plays a critical role in regulating insulin levels across various tissues in the body (1). The enzyme contains two domains, IDE-N and IDE-C, which together form a dynamic catalytic chamber essential for substrate recognition, selectivity, and processing (2,3). IDE deficiency or dysfunction has been linked to type 2 diabetes mellitus and hyperinsulinemia due to impaired insulin clearance, making IDE a potential therapeutic target for diabetes (4). IDE has also been implicated in AD pathogenesis due to substrate competition between insulin and Aβ, and may provide insight into the connection between diabetes and Alzheimer's disease (AD) (5). Alternate Names Abeta-degrading protease; FLJ35968; IDE; insulin degrading enzyme; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 118 Source/Isotype: Rabbit IgG