{"product_id":"cst-23827t","title":"CST,  23827T, IDE (F4P1J) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IDE. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, IDE (F4P1J) Rabbit Monoclonal Antibody (CST #23827) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIDE (F4P1J) Rabbit mAb recognizes endogenous levels of total IDE protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human IDE protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInsulin-degrading enzyme (IDE) is a highly conserved zinc metalloprotease responsible for clearing short bioactive peptides, including insulin, glucagon, amylin, and β-amyloid (Aβ). IDE has the highest affinity for insulin and plays a critical role in regulating insulin levels across various tissues in the body (1). The enzyme contains two domains, IDE-N and IDE-C, which together form a dynamic catalytic chamber essential for substrate recognition, selectivity, and processing (2,3). IDE deficiency or dysfunction has been linked to type 2 diabetes mellitus and hyperinsulinemia due to impaired insulin clearance, making IDE a potential therapeutic target for diabetes (4). IDE has also been implicated in AD pathogenesis due to substrate competition between insulin and Aβ, and may provide insight into the connection between diabetes and Alzheimer's disease (AD) (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAbeta-degrading protease; FLJ35968; IDE; insulin degrading enzyme; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 118\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797703807145,"sku":"23827T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-23827t","provider":"Iright","version":"1.0","type":"link"}