CST, 2383S, CEA/CD66e (CB30) Mouse Monoclonal Antibody

Monoclonal Antibody for studying CEACAM5. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CEA/CD66e (CB30) Mouse Monoclonal Antibody (CST #2383) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 42738 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CEA/CD66e (CB30) Mouse Monoclonal Antibody detects endogenous levels of CEA protein. Species Reactivity: Human Source / Purification Monoclonal antibody (Isotype: IgG1) is produced by immunizing mice with human CEA. Background Carcinoembryonic antigen (CEA), also known as CD66e or CEACAM5, is a 180-200 kDa cell surface glycoprotein whose expression is elevated in intestinal carcinomas and other tumors. CEA mediates cell adhesion, though little more is known about its biological activity. Expression of CEA is correlated with tumerogenicity (1), and it has been shown to play a role in cell migration, adhesion and invasion in culture cells, as well as in metastasis in vivo (2). Alternate Names Carcinoembryonic antigen; carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; CEA cell adhesion molecule 5; CEACAM5; CEAM5; DKFZp781M2392; Meconium antigen 100 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 180-200 Source/Isotype: Mouse IgG1 kappa