CST, 23887T, PANK1 (D2W3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PANK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PANK1 (D2W3N) Rabbit Monoclonal Antibody (CST #23887) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PANK1 (D2W3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total PANK1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro153 of human PANK1 protein. Background Pantothenate kinase 1 (PANK1) is the rate-limiting enzyme in coenzyme A (CoA) synthesis (1,2). PPARα -mediated transcriptional regulation of PANK1 plays an important role in controlling CoA levels in hepatocytes (1). Research studies indicate that PANK1 expression, along with microRNA-107, decreases in a murine macrophage model upon lipopolysaccharide (LPS) exposure in a PPARα-dependent manner (3). Additional studies show that the corresponding gene is a direct target of tumor suppressor p53 and that p53 regulates energy homeostasis through control of PANK1 expression (2). Alternate Names hPanK; hPanK1; MGC24596; PANK; PANK1; PANK1a; PANK1b; Pantothenate kinase 1; Pantothenic acid kinase 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG