{"product_id":"cst-2390s","title":"CST,  2390S, IRS-1 (59G8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IRS-1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IRS-1 (59G8) Rabbit Monoclonal Antibody (CST #2390) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 92818 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIRS-1 (59G8) Rabbit Monoclonal Antibody detects endogenous levels of total IRS-1 protein. This antibody does not cross-react with IRS-2.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser307 of human IRS-1.\n\u003cb\u003eBackground\u003c\/b\u003e\nInsulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine\/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636\/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHIRS-1; Insulin receptor substrate 1; IRS-1; IRS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 180\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797739819177,"sku":"2390S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2390s","provider":"Iright","version":"1.0","type":"link"}