CST, 2398S, Vav3 Antibody

Polyclonal Antibody for studying VAV3. Validated for Western Blotting. Highly specific and rigorously validated in-house, Vav3 Antibody (CST #2398) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Vav3 Antibody detects endogenous levels of total Vav3 protein. This antibody does not cross-react with endogenous levels of other Vav family members. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human Vav3. Antibodies are purified using protein A and peptide affinity chromatography. Background Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3). Alternate Names FLJ40431; Guanine nucleotide exchange factor VAV3; vav 3 guanine nucleotide exchange factor; vav 3 oncogene; vav guanine nucleotide exchange factor 3; VAV-3; VAV3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 98 SOURCE: Rabbit