CST, 2399S, Rap1A/Rap1B (26B4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rap1A/Rap1B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Rap1A/Rap1B (26B4) Rabbit Monoclonal Antibody (CST #2399) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rap1A/Rap1B (26B4) Rabbit Monoclonal Antibody detects endogenous levels of total Rap1A and Rap1B protein. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to the carboxy-terminal half of human Rap1A/RAP1B. Background Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6). Alternate Names C21KG; DKFZp586H0723; G-22K; GTP-binding protein smg p21A; GTP-binding protein smg p21B; K-REV; KREV-1; KREV1; RAL1B; RAP1; RAP1A; RAP1A, member of RAS oncogene family; RAP1B; RAP1B, member of RAS oncogene family; Ras family small GTP binding protein RAP1B; Ras-related protein Krev-1; Ras-related protein Rap-1A; Ras-related protein Rap-1b; RAS-related protein RAP1A; RAS-related protein RAP1B; small GTP binding protein; SMGP21 Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG