CST, 24000T, SOD3 (E8M3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SOD3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SOD3 (E8M3G) Rabbit Monoclonal Antibody (CST #24000) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SOD3 (E8M3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total SOD3 protein. This antibody cross-reacts with a band of unknown origin at approximately 18 kDa in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human SOD3 protein. Background Superoxide dismutase 3 (SOD3, EC-SOD) is a copper-containing antioxidant enzyme that plays a critical role in redox homeostasis in extracellular spaces (1). SOD3 localizes to the extracellular matrix through its C-terminal heparin binding domain, which interacts with proteoglycan on the cell surface (2). The enzyme catalyzes the dismutation of the superoxide radical (O -) to hydrogen peroxide in the extracellular space, prevents oxidative fragmentation damage of the extracellular matrix under stress conditions (3), and protects against oxidant-mediated endothelial dysfunction and tissue inflammation (4,5). SOD3 antioxidative activity preserves the bioavailability of nitric oxide (NO), providing permissive signals through the normalization of tumor vasculature, leading to enhanced tumor infiltration by effector T cells (6,7). Downregulation of SOD3 provides a selective advantage for multiple cancer types (8). A SOD3 knockout animal model displays resistance to chemotherapy response, while overexpression of SOD3 increases the tumor response to chemotherapy (9). Alternate Names EC-SOD; extracellular superoxide dismutase; Extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD3; SODE; superoxide dismutase 3; superoxide dismutase 3, extracellular; testicular tissue protein Li 175 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28, 30 Source/Isotype: Rabbit IgG