CST, 24032S, BCAR3 Antibody

Polyclonal Antibody for studying BCAR3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, BCAR3 Antibody (CST #24032) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BCAR3 Antibody recognizes endogenous levels of total BCAR3 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro526 of human BCAR3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background BCAR3 is a member of the novel SH2-containing protein (NSP) family (1). It was identified as a gene product involved in anti-estrogen resistance in the context of breast cancer (2). Like other members of this family, BCAR3 has been shown to interact with the family member, CAS. The C terminal Cdc25 homolgy domain of BCAR3 interacts tightly with the FAT domain of p130Cas (3) and promotes the association of p130cas with Src kinase (4) to activate related signaling pathways. Overexpression of BCAR3 leads to the activation of a wide range of downstream signaling proteins including PI3K, rac, PAK1, and cyclin D1 (5-7). The main role of BCAR3 is to promote cell motility and regulate cytoskeletal remodeling and adhesion through its effect on p130cas and Src kinase (8-10). BCAR3 also has been implicated in playing an inhibitory role on TGF-β/SMAD signaling, which is associated with favorable disease outcomes (11). Alternate Names AND-34; BCAR3; BCAR3 adaptor protein, NSP family member; BCAR3, NSP family adaptor protein; breast cancer anti-estrogen resistance 3; Breast cancer anti-estrogen resistance protein 3; breast cancer antiestrogen resistance 3 protein; dJ1033H22.2 (breast cancer anti-estrogen resistance 3); epididymis secretory sperm binding protein; KIAA0554; Novel SH2-containing protein 2; NSP2; SH2 domain-containing protein 3B; SH2D3B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95 SOURCE: Rabbit