{"product_id":"cst-24034w2","title":"CST,  24034W2, E-Cadherin (24E10) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 350 Conjugate)","description":"Monoclonal Antibody for studying E-Cadherin. Highly specific and rigorously validated in-house, E-Cadherin (24E10) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 350 Conjugate) (CST #24034) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nE-Cadherin (24E10) Rabbit mAb (SignalFlex™ Alexa Fluor\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the \"cadherin switch.\" N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nArc-1; BCDS1; CADH1; Cadherin; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); Cadherin E; Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120\/80; CD324; CDH1; CDHE; cell-CAM 120\/80; E-Cad\/CTF1; E-Cad\/CTF2; E-Cad\/CTF3; E-cadherin; E-cadherin 1; ECAD; epididymis secretory sperm binding protein; Epithelial cadherin; LCAM; UVO; Uvomorulin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800362373289,"sku":"24034W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-24034w2","provider":"Iright","version":"1.0","type":"link"}