CST, 24201S, SIT Antibody

Polyclonal Antibody for studying SIT. Validated for Western Blotting. Highly specific and rigorously validated in-house, SIT Antibody (CST #24201) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SIT Antibody recognizes endogenous levels of total SIT protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro75 of human SIT protein. Antibodies are purified by peptide affinity chromatography. Background SHP2-interacting transmembrane adapter protein (SIT) is a transmembrane glycoprotein that is expressed in lymphocytes as a disulfide-linked homodimer (1). The cytoplasmic domain of SIT contains multiple tyrosine residues, which when phosphorylated upon TCR engagement, recruit signaling molecules that act to negatively regulate T cell activation (1-3). Alternate Names gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP-2 interacting transmembrane adaptor protein; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold regulating transmembrane adaptor 1; Signaling threshold-regulating transmembrane adapter 1; SIT; SIT-R; SIT1; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-40 SOURCE: Rabbit