CST, 24202T, PATZ1 (F2N4R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PATZ1. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, PATZ1 (F2N4R) Rabbit Monoclonal Antibody (CST #24202) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity PATZ1 (F2N4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PATZ1 protein. This antibody may detect a 50 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PATZ1 protein. Background POZ/BTB and AT hook containing zinc finger 1 (PATZ1) is a transcription factor that regulates gene expression activation or repression depending on the cellular context (1-3). The human gene has four alternatively spliced variants that give rise to structurally similar isoforms. PATZ1 protein contains a BTB domain that facilitates protein interaction and AT hook and zinc finger domains that enable its DNA binding (4,5). PATZ1 has been shown to play key regulatory roles in embryonic development and stem cell identity, proliferation, senescence, and apoptosis (6-10). It has also been reported that PATZ1 plays a critical role in cancer cells, where it appears to carry out both oncogenic and anti-tumor activities (8-10). Notably, PATZ1 is downregulated in thyroid cancer and, thus, thought to function as a tumor suppressor in these cells (11). Alternate Names BTB-POZ domain zinc finger transcription factor; BTB/POZ domain zinc finger transcription factor; dJ400N23; MAZ-related factor; MAZR; PATZ; PATZ1; POZ (BTB) and AT hook containing zinc finger 1; POZ-, AT hook-, and zinc finger-containing protein 1; POZ-AT hook-zinc finger protein; POZ/BTB and AT hook containing zinc finger 1; Protein kinase A RI subunit alpha-associated protein; RIAZ; ZBTB19; Zinc finger and BTB domain-containing protein 19; Zinc finger protein 278; Zinc finger sarcoma gene protein; ZNF278; ZSG Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG