CST, 2423T, Notch4 (L5C5) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Notch4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Notch4 (L5C5) Mouse Monoclonal Antibody (CST #2423) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Notch4 (L5C5) Mouse Monoclonal Antibody detects levels of the transfected intracellular region of human Notch4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the Ankyrin repeat domain of human Notch4. Background Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4). Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1 (5-7). The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors (6,8). The intracellular domain of Notch4 binds to Smad3 and inhibits TGF-β signaling (9). Alternate Names FLJ16302; hNotch4; INT3; MGC74442; Neurogenic locus notch homolog protein 4; NOTC4; Notch 4; Notch 4 extracellular truncation; Notch 4 intracellular domain; Notch homolog 4; Notch homolog 4 (Drosophila); notch receptor 4; NOTCH3; NOTCH4 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 80 cleaved. 230 full-length. Source/Isotype: Mouse IgG1