CST, 24268SF, Occludin (E6B4R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying occludin. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, Occludin (E6B4R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #24268) is ready to ship. Product Usage Information This product is the carrier free version of product #91131. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 91131 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Occludin (E6B4R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total occludin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val294 of human occludin protein. Background Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability (4). Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV) (5). Alternate Names BLCPMG; hCG_1988850; Occludin; OCLN; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1; tight junction protein occludin TM4 minus Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG