CST, 24276S, CLEC2D (E4K1H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CLEC2D. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CLEC2D (E4K1H) Rabbit Monoclonal Antibody (CST #24276) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CLEC2D (E4K1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CLEC2D protein. Based on the antigen sequence, this antibody is predicted to recognize multiple isoforms of CLEC2D. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CLEC2D protein. Background C-type lectin domain family 2 member D (CLEC2D), also known as lectin-like transcript 1 (LLT1) and osteoclast inhibitory lectin (OCIL), is a type II transmembrane C-type lectin receptor primarily expressed upon immune cell activation in T cells, B cells, NK cells, and plasmacytoid and monocyte-derived dendritic cells (1-4). CLEC2D interacts with NKR-P1A (KLRB1, CD161) on NK cells and some subsets of T cells, inhibiting NK cell cytotoxicity while alternatively inducing proliferation and IFNγ production in T cells (2-4). CLEC2D is also expressed by osteoblasts, inhibiting osteoclast formation and bone resorption (5,6). Multiple isoforms of CLEC2D produced by alternative splicing have been identified (7). Alternate Names C-type lectin domain family 2 member D; C-type lectin related f; C-type lectin superfamily 2, member D; CLAX; CLC2D; CLEC2D; lectin-like NK cell receptor; Lectin-like transcript 1; LLT-1; LLT1; OCIL; osteoclast inhibitory lectin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20-30 Source/Isotype: Rabbit IgG