CST, 24357S, Eosinophil cationic protein (E6U5M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RNASE3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Eosinophil cationic protein (E6U5M) Rabbit Monoclonal Antibody (CST #24357) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:300 - 1:1200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 20523 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Eosinophil cationic protein (E6U5M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Eosinophil cationic protein. This antibody does not cross-react with Eosinophil-derived neurotoxin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly130 of human Eosinophil cationic protein. Background Eosinophil cationic protein (ECP, RNASE3) is a member of the ribonuclease superfamily which has both cytotoxic and noncytotoxic properties (1-4). ECP is primarily produced and stored in the secondary granules of eosinophils, where it is involved in host defense against parasites, bacteria, and viruses (1-4). It has also been detected in the primary granules of circulating neutrophils, and can be synthesized by stimulated neutrophils (3,4). ECP is often used as a marker for eosinophilic disease and is measurable in samples such as serum, bronchoalveolar lavage, and nasal secretions (4). A large number of clinical studies are focused on the role of ECP in eosinophil-related disorders, particularly in asthma (5). Alternate Names cytotoxic ribonuclease; ECP; Eosinophil cationic protein; RAF1; Ribonuclease 3; ribonuclease A family member 3; ribonuclease, RNase A family, 3; ribonuclease, RNase A family, 3 (eosinophil cationic protein); RNase 3; RNASE3; RNS3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG