CST, 24510SF, CD200 (E5I9V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD200. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD200 (E5I9V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #24510) is ready to ship. Product Usage Information This product is the carrier-free version of product #23451. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 23451 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD200 (E5I9V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD200 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human CD200 recombinant protein and reacts with an epitope surrounding Ala164. Background CD200 (OX2) and CD200R (OX2R) are membrane glycoprotein members of the Ig superfamily (1-3). CD200 is expressed by a range of cells, including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells, while its receptor, CD200R, is found on myeloid and T cells (1-5). Interaction between CD200 and CD200R downregulates macrophage function and plays a role in immunosuppression and regulation of anti-tumor immune responses (3-7). Alternate Names antigen identified by monoclonal antibody MRC OX-2; CD200; CD200 antigen; CD200 molecule; MOX1; MOX2; MRC; MRC OX-2 antigen; OX-2; OX-2 membrane glycoprotein; OX2G Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-50 Source/Isotype: Rabbit IgG