CST, 24584S, PGAM5 (Long Isoform) Antibody

Polyclonal Antibody for studying PGAM5. Validated for Western Blotting. Highly specific and rigorously validated in-house, PGAM5 (Long Isoform) Antibody (CST #24584) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PGAM5 (Long Isoform) Antibody recognizes endogenous levels of total PGAM5 long isoform protein. This antibody specifically recognizes the PGAM5 long isoform (UniProt #Q96HS1-1) and does not recognize the PGAM5 short isoform (UniProt #Q96HS1-2). Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PGAM5 long isoform (UniProt #Q96HS1-1). Antibodies are purified by protein A and peptide affinity chromatography. Background Phosphoglycerate mutase family 5 (PGAM5) is a mitochondrial serine/threonine protein phosphatase (1,2). PGAM5 is required for necrosis induced by TNF-α or reactive oxygen species (2). In addition, PGAM5 dephosphorylates FUNDC1 to induce mitophagy under mitochondrial stresses (3). PGAM5 is also essential for RIPK1-mediated mitophagy activation during extracellular matrix (ECM) detachment of cancer cells (4). Furthermore, PGAM5 is cleaved by the protease presenilin-associated rhomboid-like protein (PARL) in response to mitochondrial stresses (such as induced by carbonyl cyanide -chlorophenylhydrazone (CCCP) treatment). The cleaved PGAM5, released from the mitochondrial membrane, dephosphorylates β-catenin in the cytosol leading to its stabilization. Increased levels of β-catenin stimulate mitochondrial biogenesis (5). Alternate Names Bcl-XL-binding protein v68; BXLBV68; MGC5352; PGAM family member 5, mitochondrial serine/threonine protein phosphatase; PGAM family member 5, serine/threonine protein phosphatase, mitochondrial; PGAM5; Phosphoglycerate mutase family member 5; Serine/threonine-protein phosphatase PGAM5, mitochondrial Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 28, 30 SOURCE: Rabbit