CST, 24595S, CD206/MRC1 (E6T5J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MRC1 mouse. Validated for WB,WB,IP,IHC,IHC,IF,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, CD206/MRC1 (E6T5J) Rabbit Monoclonal Antibody (CST #24595) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:400 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 87887 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD206/MRC1 (E6T5J) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD206/MRC1 protein. This antibody recognizes mouse CD206/MRC1 protein and is also reactive with human CD206/MRC1; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Instead, CD206/MRC1 (E2L9N) Rabbit mAb #91992 is recommended for IHC analysis of human tissue samples. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD206/MRC1 protein. Background The mannose receptor (MR/CLEC13D/CD206/MMR/MRC1/Macrophage mannose receptor 1) is an endocytic receptor expressed by populations of dendritic cells, macrophages, and nonvascular endothelium (1). The mannose receptor is a heavily glycosylated type I transmembrane protein with three types of extracellular domains and a short carboxy-terminal cytoplasmic domain with no apparent signaling motif (2-4). The extracellular portion of the protein is made up of a CR domain, which binds sulfated glycans, an FNII domain, which binds collagens, and eight C-type lectin domains, which bind carbohydrates containing mannose, fucose, or GlcNAc (4-7). The receptor recycles between the plasma membrane and early endosomes (8). Functions include a role in antigen cross-presentation, clearance of endogenous proteins, pathogen detection, and trafficking through lymphatic vessels (9-12). Alternate Names AW259686; CD206; Macrophage mannose receptor 1; mannose receptor, C type 1; MMR; MR; Mrc1; OTTMUSP00000012205 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 190-250 Source/Isotype: Rabbit IgG