CST, 2460S, AMPA Receptor (GluA2/3/4) Antibody

Polyclonal Antibody for studying GluR3/GluR4/GluR2. Validated for Western Blotting. Highly specific and rigorously validated in-house, AMPA Receptor (GluA2/3/4) Antibody (CST #2460) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity AMPA Receptor (GluA2/3/4) Antibody detects endogenous levels of total GluA2/3/4 protein. This antibody may also detect GluA1. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser720 of human GluA2. Antibodies are purified by protein A and peptide affinity chromatography. Background AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluA 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluA 2-containing AMPARs, AMPARs that lack GluA 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer's, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). Alternate Names AMPA-selective glutamate receptor 2; AMPA-selective glutamate receptor 3; AMPA-selective glutamate receptor 4; dJ1171F9.1; GluA2; GluA3; GluA4; GluR-2; GluR-3; GluR-4; GluR-B; GluR-C; GluR-D; GluR-K2; GluR-K3; GLUR2; GLUR3; GLUR4; GLUR4C; GLURB; GLURC; GLURD; glutamate ionotropic receptor AMPA type subunit 2; glutamate ionotropic receptor AMPA type subunit 3; glutamate ionotropic receptor AMPA type subunit 4; Glutamate receptor 2; Glutamate receptor 3; Glutamate receptor 4; glutamate receptor C; Glutamate receptor ionotropic, AMPA 2; Glutamate receptor ionotropic, AMPA 3; Glutamate receptor ionotropic, AMPA 4; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotrophic, AMPA 4; glutamate receptor, ionotropic, AMPA 2; glutamate receptor, ionotropic, AMPA 3; glutamate receptor, ionotropic, AMPA 4; GRIA2; GRIA3; GRIA4; HBGR2; MRX94; MRXSW; NEDLIB; NEDSGA Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit