{"product_id":"cst-2466s","title":"CST,  2466S, Cdc42 (11A11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Cdc42. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cdc42 (11A11) Rabbit Monoclonal Antibody (CST #2466) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCdc42 (11A11) Rabbit Monoclonal Antibody detects endogenous levels of total Cdc42 protein. This antibody does not cross-react with other small GTPases.\nSpecies Reactivity: Human, Mouse, Rat, Bovine\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys135 of human Cdc42.\n\u003cb\u003eBackground\u003c\/b\u003e\nRac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac\/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCDC42; CDC42Hs; Cell division control protein 42 homolog; cell division cycle 42; cell division cycle 42 (GTP binding protein, 25kDa); dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K; G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein, 25kD; small GTP binding protein CDC42; TKS\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R B\nSENSITIVITY: Endogenous\nMW (kDa): 21\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797852377257,"sku":"2466S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2466s","provider":"Iright","version":"1.0","type":"link"}