CST, 2468S, PTCH1 (C53A3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PTCH1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PTCH1 (C53A3) Rabbit Monoclonal Antibody (CST #2468) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PTCH1 (C53A3) Rabbit Monoclonal Antibody detects immunoprecipitated or transfected levels of PTCH1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1307 of human PTCH1. Background Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10). PTCH1 itself is a target of Hedgehog signaling (11), with elevated PTCH1 expression as a surrogate marker for Hedgehog pathway activation (12-14). Alternate Names BCNS; FLJ26746; FLJ42602; HPE7; NBCCS; patched 1; patched homolog 1 (Drosophila); Protein patched homolog 1; PTC; PTC1; PTCH; PTCH protein -10; PTCH protein +12b; PTCH protein +4'; PTCH1; PTCH11 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 180-210 Source/Isotype: Rabbit IgG lambda