{"product_id":"cst-24846t","title":"CST,  24846T, Phospho-AAK1 (Thr620) (F6J5X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying AAK1 (Thr620) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-AAK1 (Thr620) (F6J5X) Rabbit Monoclonal Antibody (CST #24846) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-AAK1 (Thr620) (F6J5X) Rabbit Monoclonal Antibody recognizes endogenous levels of AAK1 protein only when phosphorylated at Thr620.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr620 of human AAK1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAdaptor-associated kinase 1 (AAK1) is a member of the Ark1\/Prk1 family of serine\/threonine kinases (1). AAK1 is enriched in synaptosomal preparations and modulates clathrin-dependent endocytosis, a process that is important in synaptic vesicle recycling and receptor-mediated endocytosis. AAK1, together with clathrin and clathrin-adaptor protein AP-2, forms a signaling complex at the cell membrane. AAK1-dependent phosphorylation of the mu-2 subunit of AP-2 enhances endocytosis efficiency (2,3). AAK1 is known to promote neuregulin\/ErbB4 internalization to regulate neurotrophic signaling. Inhibition of AAK1 activity promotes cell surface expression of neuregulin\/ErbB4, cell-bound neurotrophic factors that are implicated in brain development and synaptic plasticity (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAAK1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; KIAA1048; MGC138170; MGC164568; MGC164570\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 142\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797889798313,"sku":"24846T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-24846t","provider":"Iright","version":"1.0","type":"link"}