{"product_id":"cst-24903s","title":"CST,  24903S, NFI-B (E5L7U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NFI-B. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, NFI-B (E5L7U) Rabbit Monoclonal Antibody (CST #24903) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNFI-B (E5L7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total NFI-B protein. Based on antigen sequence, the antibody is predicted to detect isoform 1 and isoforms 4-6 of NFI-B. Based on sequence homology, this antibody is not predicted to cross-react with other NFI family members. Species cross-reactivity for IF-IC is human only.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro404 of human NFI-B protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNFI-B belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). Research studies have demonstrated that NFI-B plays a crucial role in different aspects of tumor development, acting as an oncogene or tumor suppressor in different types of tumors and participating in tumor-associated fusion gene formation (4-7). Moreover, NFI-B has been shown to promote epithelial-mesenchymal transition and metastatic ability of cancer cells through a number of mechanisms, including upregulating SNAI1 expression in colorectal cancer (8), stabilizing chromatin accessibility in small cell lung cancer (9), and modulating the ZEB1 axis in melanoma (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCAAT-box-binding transcription factor; CTF; HMGIC\/NFIB; MACID; NF-I\/B; NF1-B; NFI-B; NFI-RED; NFIB; NFIB2; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1\/B; nuclear factor I B; Nuclear factor I\/B; TGGCA-binding protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 50-75\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797890093225,"sku":"24903S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-24903s","provider":"Iright","version":"1.0","type":"link"}