{"product_id":"cst-24965s","title":"CST,  24965S, CD11b\/ITGAM (M1\/70) Rat Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying CD11b\/ITGAM. Validated for Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD11b\/ITGAM (M1\/70) Rat Monoclonal Antibody (PE Conjugate) (CST #24965) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal flow cytometry results, we recommend 0.125 μg of antibody per test.\nFlow Cytometry (Fixed\/Permeabilized): 1:160\nFlow Cytometry (Live): 1:160\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD11b\/ITGAM (M1\/70) Rat Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD11b protein. This antibody conjugate detects an epitope within the extracellular domain.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nCluster of differentiation molecule 11b (CD11b)\/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4). The M1\/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC) (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nantigen CD11b (p170); CD11 antigen-like family member B; CD11B; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; Integrin alpha-M; integrin subunit alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITAM; ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rat IgG2b kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800364339369,"sku":"24965S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-24965s","provider":"Iright","version":"1.0","type":"link"}