{"product_id":"cst-25000s","title":"CST,  25000S, USB1 (F4R5D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying USB1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, USB1 (F4R5D) Rabbit Monoclonal Antibody (CST #25000) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUSB1 (F4R5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total USB1 protein.\nSpecies Reactivity: Human, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human USB1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nU6 biogenesis 1 (USB1), also known as MPN1, is a 3'-5' RNA exonuclease that removes uridines and adenosines from the 3' end of U6 snRNA, creating a 2', 3'-cyclic phosphate formation, which protects U6 snRNA from degradation by the nuclear exosome (1-3). USB1 measures the U6 oligo(U) tail by reading the A102 nucleotide position and pauses five uridine residues downstream (3). Loss of USB1 results in a rare condition called poikiloderma with neutropenia, where U6 snRNA is unstable and contains aberrantly long and oligoadenylated 3' ends, resulting in splicing dysfunction (1,4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC16orf57; chromosome 16 open reading frame 57; CP057; FLJ13154; hUsb1; HVSL motif containing 1; HVSL1; hypothetical protein LOC79650; Mpn1; mutated in poikiloderma with neutropenia protein 1; PN; putative U6 snRNA phosphodiesterase; U six biogenesis 1; U6 snRNA biogenesis 1; U6 snRNA biogenesis phosphodiesterase 1; U6 snRNA phosphodiesterase; U6 snRNA phosphodiesterase 1; UPF0406 protein C16orf57; USB1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 25, 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797891076265,"sku":"25000S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-25000s","provider":"Iright","version":"1.0","type":"link"}