{"product_id":"cst-2502s","title":"CST,  2502S, Vav1 Antibody","description":"Polyclonal Antibody for studying VAV1. Validated for Western Blotting,Simple Western™,Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Vav1 Antibody (CST #2502) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nFlow Cytometry (Fixed\/Permeabilized): 1:25\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nVav1 Antibody detects endogenous levels of total Vav1 protein. This antibody does not cross-react with Vav2, Vav3 or CelVav.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr174 of human Vav. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nVav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho\/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho\/Rac (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\noncogene vav; Proto-oncogene vav; VAV; vav 1 guanine nucleotide exchange factor; vav 1 oncogene; vav guanine nucleotide exchange factor 1; vav proto-oncogene; VAV1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 95\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797891469481,"sku":"2502S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2502s","provider":"Iright","version":"1.0","type":"link"}