{"product_id":"cst-25081s","title":"CST,  25081S, PTMScan® Phospho-Ser\/Thr Motif [pS\/T] Kit","description":"PTMScan for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan ® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS\/MS analysis. CST recommends the use of PTMScan ® IAP Buffer #9993 included in the kit.\n\u003cb\u003eStorage\u003c\/b\u003e\nAntibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: PTMScan\n\u003cb\u003eBackground\u003c\/b\u003e\nAs an integral part of the machinery of cellular function, proteins undergo regulation by a variety of post-translational modifications. One of the most prevalent and widely studied PTMs is Serine\/Threonine phosphorylation. A few prominent kinases targeting a handful of substrate consensus motifs account for a majority of the tens of thousands of known and predicted sites on more than 13,000 human proteins (1-3). Cell Signaling Technology has developed phospho-Ser\/Thr motif antibodies for proteomic profiling of kinase substrates. These include, among others, substrates of Akt, AMPK, MAPK, CDK, PKA, PKC, and CKII.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nPan-Phospho","brand":"CST","offers":[{"title":"Default Title","offer_id":46800793993385,"sku":"25081S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-25081s","provider":"Iright","version":"1.0","type":"link"}