{"product_id":"cst-25177s","title":"CST,  25177S, ATF-1 Antibody","description":"Polyclonal Antibody for studying ATF-1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ATF-1 Antibody (CST #25177) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nATF-1 Antibody recognizes endogenous levels of total ATF-1 protein. This antibody may detect bands of unknown identity at 130-150 kDa.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ATF-1 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nActivating transcription factor 1 (ATF-1) is a bZIP transcription factor that is a member of the cyclic AMP (cAMP)-response element-binding protein (CREB) family. Like other members of the CREB family, ATF-1 mediates signals from numerous physiological stimuli and activates target genes through cAMP-response elements, resulting in the regulation of a broad array of cellular responses. ATF-1 can be phosphorylated at Ser63 by protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), and cyclin-dependent kinase 3 (CDK3), which recruits CBP\/p300 and enhances transcriptional activity (1-3). ATF-1 expression levels have been found to be elevated in lymphomas and metastatic melanoma cells, where it contributes to tumor growth (4,5). The gene fusion of with , which encodes for the EWSR1-ATF1 oncoprotein, is also implicated in a variety of cancers, most notably in clear cell sarcoma (CCS) (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nActivating transcription factor 1; ATF-1; ATF1; cAMP-dependent transcription factor ATF-1; Cyclic AMP-dependent transcription factor ATF-1; EWS-ATF1; FUS\/ATF-1; Protein TREB36; TREB36\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797892386985,"sku":"25177S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-25177s","provider":"Iright","version":"1.0","type":"link"}