{"product_id":"cst-25229l","title":"CST,  25229L, CD4 (D7D2Z) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD4 mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 3 sizes. Highly specific and rigorously validated in-house, CD4 (D7D2Z) Rabbit Monoclonal Antibody (CST #25229) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nIHC Leica Bond: 1:50 - 1:200\nImmunohistochemistry (Paraffin): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 27520 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD4 (D7D2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse and rat CD4 protein. Non-specific staining in mouse kidney and liver has been observed.\nSpecies Reactivity: Mouse, Rat, Hamster\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala232 of mouse CD4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCd4; CD4 antigen; L3T; L3T4; Ly-; Ly-4; OTTMUSP00000027399; T-cell differentiation antigen L3T4; T-cell surface antigen T4\/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4\/Leu-3) (T-cell differentiation antigen L3T4)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R Hm\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801054498985,"sku":"25229L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-25229l","provider":"Iright","version":"1.0","type":"link"}