{"product_id":"cst-2561s","title":"CST,  2561S, Phospho-CDK2 (Thr160) Antibody","description":"Polyclonal Antibody for studying Cdk2 (Thr160) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-CDK2 (Thr160) Antibody (CST #2561) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-CDK2 (Thr160) Antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160. This antibody weakly cross-reacts with cdc2 phosphorylated at Thr161. This antibody does not cross-react with other phosphorylated cyclin dependent kinases.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr160 of human CDK2. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1\/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2\/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2\/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncdc2-related protein kinase; CDK2; CDKN2; cell devision kinase 2; Cell division protein kinase 2; cyclin dependent kinase 2; Cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 33\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797895958697,"sku":"2561S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2561s","provider":"Iright","version":"1.0","type":"link"}