{"product_id":"cst-2568l","title":"CST,  2568L, Phospho-LRP6 (Ser1490) Antibody","description":"Polyclonal Antibody for studying LRP6 (Ser1490) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-LRP6 (Ser1490) Antibody (CST #2568) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-LRP6 (Ser1490) Antibody detects endogenous levels of LRP6 protein when phosphorylated at serine 1490. This antibody is expected, although not yet confirmed, to also detect endogenous levels of LRP5 protein when phosphorylated at serine 1503 (corresponding to serine 1490 in LRP6).\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1490 of human LRP6. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nLRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt\/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nADCAD2; FLJ90062; FLJ90421; LDL receptor related protein 6; low density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; LRP-6; LRP6; STHAG7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 210\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797896581289,"sku":"2568L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2568l","provider":"Iright","version":"1.0","type":"link"}