{"product_id":"cst-25729t","title":"CST,  25729T, PPP1R14B\/PHI-1 (F6O6F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PPP1R14B. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PPP1R14B\/PHI-1 (F6O6F) Rabbit Monoclonal Antibody (CST #25729) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:400 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPPP1R14B\/PHI-1 (F6O6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total PPP1R14B\/PHI-1 protein. This antibody does not cross-react with PPP1R14A\/CPI-17. Non-specific staining was observed in mouse brain, spleen, and liver by immunohistochemistry.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val50 of human PPP1R14B\/PHI-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPhosphatase holoenzyme inhibitor (PHI-1), encoded by the protein phosphatase 1 (PP1) regulatory subunit 14B gene ( ), belongs to a family of proteins, including PPP1R14A\/CPI-17, PPP1R14C\/KEPI, and PPP1R14D\/GEPI that each specifically inhibits a subset of PP1 holoenzymes. PHI-1 is highly conserved among eukaryotes and ubiquitously expressed at low levels across tissue types, but it is overexpressed in a wide array of cancer types, including colon and lung adenocarcinomas (1,2). PHI-1 also displays a proteostatic function, both directly and indirectly, on several targets in the MAPK signaling pathways. In hepatocellular carcinoma (HCC) cells, PHI-1 bound to, stabilized, and increased phosphorylation of p90RSK at Ser363\/Ser380, and PHI-1 knockdown inhibited HCC cell growth (3). In HEK 293 cells, PHI-1 knockdown stabilized c-Raf protein and increased downstream ERK1\/ERK2 activation (4). PHI-1 phosphorylation at Thr57 increases its inhibitory activity toward PP1 50-fold, and it has been established as a PKC and ILK substrate (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nPHI-1; phospholipase C beta-3 neighboring gene protein; Phospholipase C-beta-3 neighbouring gene protein; PLCB3N; PNG; PP14B; PPP1R14B; protein phosphatase 1 regulatory inhibitor subunit 14B; Protein phosphatase 1 regulatory subunit 14B; protein phosphatase 1, regulatory (inhibitor) subunit 14B; SOM172\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 19\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797896777897,"sku":"25729T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-25729t","provider":"Iright","version":"1.0","type":"link"}