{"product_id":"cst-2580s","title":"CST,  2580S, Phospho-IRS-1 (Ser332\/336) Antibody","description":"Polyclonal Antibody for studying IRS-1 (Ser337\/Ser341) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-IRS-1 (Ser332\/336) Antibody (CST #2580) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-IRS-1 (Ser332\/336) Antibody detects transfected levels of IRS-1 when phosphorylated at Ser332\/336. This antibody also detects IRS-1 protein when singly phosphorylated at Ser332 or Ser336 of human IRS-1. This antibody does not cross-react with other related phosphoproteins.\nSpecies Reactivity: Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser332\/336 of mouse IRS-1 (equivalent to Ser337\/341 of human IRS-1). Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nInsulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine\/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636\/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). GSK-3-mediated IRS-1 serine phosphorylation leads to inhibition of insulin-stimulated IRS-1 signaling. Ser332 and Ser336 of IRS-1 are situated in a glycogen synthase kinase-3 (GSK-3) consensus motif (SXXXS), and it has been shown that Ser332 is the actual GSK-3 phosphorylation site while Ser336 provides a \" priming\" site necessary for GSK-3 action (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHIRS-1; Insulin receptor substrate 1; IRS-1; IRS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: R\nSENSITIVITY: Transfected Only\nMW (kDa): 180\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800181330089,"sku":"2580S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2580s","provider":"Iright","version":"1.0","type":"link"}