{"product_id":"cst-25816s","title":"CST,  25816S, FoxK1 (E4D1V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FOXK1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, FoxK1 (E4D1V) Rabbit Monoclonal Antibody (CST #25816) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFoxK1 (E4D1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxK1 protein. A weak band of unknown identity is detected at 80 kDa.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val707 of human FoxK1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nForkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA-binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech\/language disorders, and cancer (3,4). FoxK1 belongs to a subfamily of Fox proteins along with FoxK2. FoxK1 functions as a transcriptional repressor of downstream target genes, including , , , , and (5-7). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. The β isoform has a truncated C terminus compared to the α isoform. The two isoforms of FoxK1 show reciprocal expression patterns during muscle regeneration. FoxK1-β is expressed mainly in quiescent satellite cells (8), whereas FoxK1-α is the dominant isoform in proliferating myoblasts from activated satellite cells (9). Both isoforms of FoxK1 jointly regulate proliferation and differentiation of myogenic stem cells during development and muscle regeneration (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ14977; forkhead box K1; Forkhead box protein K1; FOXK1; FOXK1L; MNF; Myocyte nuclear factor\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 97\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797898088617,"sku":"25816S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-25816s","provider":"Iright","version":"1.0","type":"link"}