{"product_id":"cst-2587s","title":"CST,  2587S, TORC1\/CRTC1 (C71D11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TORC1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TORC1\/CRTC1 (C71D11) Rabbit Monoclonal Antibody (CST #2587) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTORC1\/CRTC1 (C71D11) Rabbit Monoclonal Antibody recognizes endogenous levels of total TORC1 (CRTC1) protein.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val463 of human TORC1 (CRTC1) protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGlucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein CRTC2 (CREB-regulated transcription coactivator 2)\/TORC2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, CRTC2\/TORC2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of CRTC2\/TORC2 and its dissociation from 14-3-3 proteins. Dephosphorylated CRTC2\/TORC2 enters the nucleus to promote CREB-dependent transcription. CRTC2\/TORC2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). CRTC2\/TORC2-related proteins CRTC1\/TORC1 and CRTC3\/TORC3 also act as CREB co-activators (2,3). CRTC1\/TORC1, CRTC2\/TORC2 and CRTC3\/TORC3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats (6,7). CRTC1\/TORC1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions (8). When these cells are exposed to cAMP or a calcium activator, CRTC1\/TORC1 is dephosphorylated and translocates into the nucleus (8). CRTC1\/TORC1 is essential for energy balance and fertility (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCREB regulated transcription coactivator 1; CREB-regulated transcription coactivator 1; CRTC1; FLJ14027; KIAA0616; Mam-2; MAML2; Mastermind-like protein 2; MECT1; mucoepidermoid carcinoma translocated 1; Mucoepidermoid carcinoma translocated protein 1; TORC-1; TORC1; Transducer of CREB protein 1; transducer of regulated cAMP response element-binding protein (CREB) 1; Transducer of regulated cAMP response element-binding protein 1; WAMTP1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 78\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801055154345,"sku":"2587S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2587s","provider":"Iright","version":"1.0","type":"link"}