{"product_id":"cst-2599s","title":"CST,  2599S, Phospho-FoxO1 (Thr24)\/FoxO3a (Thr32)\/FoxO4 (Thr28) (4G6) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FOXO4 (Thr32) phosphate\/FoxO3a (Thr32) phosphate\/FoxO1a (Thr24) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-FoxO1 (Thr24)\/FoxO3a (Thr32)\/FoxO4 (Thr28) (4G6) Rabbit Monoclonal Antibody (CST #2599) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-FoxO1 (Thr24)\/FoxO3a (Thr32)\/FoxO4 (Thr28) (4G6) Rabbit Monoclonal Antibody detects endogenous levels of FoxO1 when phosphorylated at Thr24, of FoxO3a when phosphorylated at Thr32 or FoxO4 when phosphorylated at Thr28.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAF6q21; AF6q21 protein; AFX; AFX1; DKFZp781A0677; FKH1; FKHR; FKHRL1; FKHRL1P2; Fork head domain transcription factor AFX1; forkhead box O1; forkhead box O1A (rhabdomyosarcoma); forkhead box O3; forkhead box O3A; forkhead box O4; Forkhead box protein O1; Forkhead box protein O1A; Forkhead box protein O3; Forkhead box protein O4; forkhead homolog (rhabdomyosarcoma) like 1; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO1; FOXO1A; FOXO2; FOXO3; FOXO3A; FOXO4; MGC120490; MGC12739; MGC31925; MLLT7; myeloid\/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 65, 78 to 82, 95\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797934624937,"sku":"2599S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2599s","provider":"Iright","version":"1.0","type":"link"}