{"product_id":"cst-26080s","title":"CST,  26080S, SHMT1 (F2H4H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SHMT1. Validated for Western Blotting,Simple Western™,Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, SHMT1 (F2H4H) Rabbit Monoclonal Antibody (CST #26080) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nFlow Cytometry (Fixed\/Permeabilized): 1:1200 - 1:4800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSHMT1 (F2H4H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SHMT1 protein. This antibody does not cross-react with SHMT2 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SHMT1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSerine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5,10-methylene-THF (1,2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n14 kDa protein; CSHMT; cytoplasmic serine hydroxymethyltransferase; GLYC; Glycine hydroxymethyltransferase; MGC15229; MGC24556; serine hydroxymethyltransferase 1; serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT; SHMT1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797935411369,"sku":"26080S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-26080s","provider":"Iright","version":"1.0","type":"link"}