{"product_id":"cst-2637s","title":"CST,  2637S, BTAF1 Antibody","description":"Polyclonal Antibody for studying TAF172. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, BTAF1 Antibody (CST #2637) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBTAF1 Antibody recognizes endogenous levels of total BTAF1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala220 of human BTAF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nB-TATA binding protein-associated factor 1 (BTAF1) is a human homolog of the yeast protein modifier of transcription 1 (MOT1) (1). BTAF1 negatively regulates transcription through interaction with TATA binding protein (TBP), an important component of the B-TFIID complex (2,3). BTAF1 is a member of the SNF2 family of ATPases that utilizes ATP to remove TBP from B-TFIID, thereby preventing formation of the transcription pre-initiation complex (PIC) (3-5). BTAF1 actively removes TBP from DNA, which prevents non-specific TBP-DNA interactions and promotes disassembly of inactive forms of the PIC. This activity is thought to be critical for maintaining a pool of free TBP that is necessary for the assembly of the PIC during transcription activation (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATP-dependent helicase BTAF1; B-TFIID TATA-box binding protein associated factor 1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae); KIAA0940; MGC138406; MOT1; Mot1 homolog; TAF-172; TAF(II)170; TAF172; TAFII170; TATA-binding protein-associated factor 172; TBP-associated factor 172\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797937180841,"sku":"2637S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2637s","provider":"Iright","version":"1.0","type":"link"}