{"product_id":"cst-2678s","title":"CST,  2678S, IKK beta (L570) Antibody","description":"Polyclonal Antibody for studying IKK-beta. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IKK beta (L570) Antibody (CST #2678) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIKK beta (L570) Antibody detects endogenous levels of total IKKβ protein.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine, Guinea Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu570 of IKKβ protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe NF-κB\/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkappaB kinase; IKBKB; IKK-B; IKK-beta; IKK2; IKKB; IMD15; IMD15A; IMD15B; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa B kinase subunit beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk B GP\nSENSITIVITY: Endogenous\nMW (kDa): 87\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797940916393,"sku":"2678S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2678s","provider":"Iright","version":"1.0","type":"link"}