{"product_id":"cst-26836t","title":"CST,  26836T, Fibronectin\/FN1 (E5H6X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FN1. Validated for WB,IP,IHC,IHC,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, Fibronectin\/FN1 (E5H6X) Rabbit Monoclonal Antibody (CST #26836) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nIHC Leica Bond: 1:400 - 1:1600\nImmunohistochemistry (Paraffin): 1:100 - 1:400\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44800 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFibronectin\/FN1 (E5H6X) Rabbit Monoclonal Antibody recognizes endogenous levels of total Fibronectin\/FN1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a protein fragment corresponding to residues near the carboxy terminus of human Fibronectin\/FN1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nFibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAnastellin; CIG; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; epididymis secretory sperm binding protein; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF; Ugl-Y1; Ugl-Y2; Ugl-Y3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 300\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797941407913,"sku":"26836T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-26836t","provider":"Iright","version":"1.0","type":"link"}